Vol. 16,No. 2, April 2017
Responses of growth rates and growth hormone levels of African catfish (Clarias gariepinus) to stocking density
Description:Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, P. R. China Corresponding author's Email: xiaomeiwem@yahoo.com
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Abstract
In order to estimate responses of growth rate and growth hormone ofClarias gariepinus to stocking density, fish (initial weight 30.71 ± 0.89 g) were stocked in 120-L tanks at densities of 35, 65, 95, and 125 kg/m3 for 60 days. On day 30 and 60, the fish growth rates were measured, pituitary growth hormone (GH) mRNA levels and serum GH concentrations were examined by real-time qPCR and ELISA technique, respectively. From day 0 to day 30, there were significant decreases in specific growth rates and relative weight gains at higher densities (95 and 125 kg/m3) compared to that at lower densities (35 and 65 kg/m3), whereas no significant effects of stocking density on the two indexes during the day 30−60 were detected. On day 30, GH mRNA levels were unaffected by stocking density, while on day 60, GH mRNA levels were significantly lower at the density of 125 kg/m3 than in the two lower densities. Serum GH concentration increased with decreasing stocking densities but only significantly between 35 kg/m3 and the other treatments on day 30. However, there were no significant differences between two lower densities as well as between 65 kg/m3 and 95 kg/m3 treatments on day 60. These results implied that effect of stocking density on SGR and RWG ofC. gariepinus weren’t uniform in different growth periods and a weak positive correlation was observed between growth rate and GH inC. gariepinus in the present study.
Keywords:Clarias gariepinus, Stocking density, Specific growth rate, Relative weight gain, Growth hormone, Real-time qPCR, ELISA
Author:Wang X.M.; Gao J.W.; Xu M.; Mo B.L.; Dai W.; Chen CHh.X.
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