Vol. 16,No. 2, April 2017
Dispersal patterns of endogenous bacteria among grass carp (Ctenopharyngodon idellus) guts
Description:1- Key Laboratory of Biodiversity and Conservation of Aquatic Organisms, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. 2- Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China. 3- State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, China. 4- Animal Science and Technology College, Hunan Agricultural University, Changsha 410128, China. 5- Yuanjiang Bureau of Aquatic Products, Yuanjiang 413100, China. 6- Graduate School of the Chinese Academy of Sciences, Beijing 100039, China. * Corresponding authors' Email: yhyu@ihb.ac.cn
The formation and regulation of vertebrate endogenous intestinal microbiota has been widely studied as the microbiota plays a crucial role in the host nutrition, development, and health. Despite the importance of microbiota for host health, it is still unclear whether the endogenous intestinal microorganisms are genetically distinct or whether they are genetically related with each other in different host individuals. In the present study, the dispersal situation of the endogenous intestinal bacteria in grass carp was investigated by constructing bacterial 16S rRNA gene clone libraries. The results indicate that the bacteria harbored in the grass carp gut could be separated into the following two groups: a- the private operational taxonomic units (OTUs), which includeCetobacterium somerae,Aeromonas jandaei,Citrobacter freundii,Achromobacter xylosoxidans andBacteroides species; b- the shared OTUs, which includeVibrio cholerae,Plesiomonas shigelloides andPasteurella speices. The results obtained in this investigation provide valuable information for assessing the mechanism of spread of the endogenous intestinal bacteria, especially the pathogenic ones. However, the mechanisms involved in different modes of bacterial dispersal in the grass carp gut still require further research.
Keywords: Dispersal barrier, Intestinal bacteria, Clone library, Grass carp
Author:Ni J.J.1,2,3,6; Yan Q.Y.1; Yu Y.H.*; Wu H.H.4; Chen F.5
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