Vol. 16,No. 1, January 2017
Determination of the inhibitory effects of microdiets used in routine commercial feeding protocols on protease activities of Argyrosomus regius (Asso, 1801) larva
Description:1-Department of Basic Science, Eğirdir Fisheries Faculty, Süleyman Demirel University, Isparta, 32040, Turkey. 2- Department of Aquaculture, Eğirdir Fisheries Faculty, Süleyman Demirel University, Isparta, 32040, Turkey. 3-Department of Aquaculture, Marine Science and Technology Faculty, İskenderun Technical University, İskenderun, 31200, Turkey *Corresponding author’s Email: gurkandiken@sdu.edu.tr
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Abstract
The aim of this study was to determine the inhibitory effects of feed ingredients on protease activities ofArgyrosomus regiuslarvae usingin vitrotechniques.A. regiuslarvae fed on a commercial feeding procedure were sampled thirteen times, during the sampling period (from 3 to 32 days after hatching (DAH)). The differences observed in protease activities of meagre larvae during the sampling period were statistically significant (p<0.05). The lowest and highest protease activities of meagre larvae were 5.95±0.6 U/mg protein (15 DAH) and 211.21±12.56 U/mg protein (7 DAH), respectively. The fluctuations observed in protease activities ofA. regius larvae were between 10 DAH and 32 DAH. Commercial diets such as Orange Start-S (100-200µ), Orange Start-L (200-300µ), Orange Nurse-XS (300-500µ), Orange Grow-S (300-500µ) and Orange Grow-L (500-800µ) caused the inhibitions on protease activities in meagre larvae to range from 16 to 32 DAH. The results point to the inadequacy of commercial diets such as Orange Grow-S, Orange Grow-L and suitability of Orange Start-S, Orange Start-L, Orange Nurse-XS for feeding meagre larvae during the weaning stage. For the mass production of quality juveniles, future studies should take into account the inhibitory effects of commercial diets and feed ingredients before the manufacturing process. A similar approach may be used to determine the most suitable commercial diets for use during the weaning stages of marine fish larvae to obtain the best growth performance and survival.
Keywords: Meagre, Argyrosomus regius, Protease activities, Protease inhibitions, Commercial diets,in vitro
Author:Diken G.1*; Demir O.2; Naz M.3
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