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Molecular identification of some causative agents of warm-water streptococcosis by M-PCR in cultured rainbow trout, Chaharmahal - Bakhtiari Province, Iran
Description:1- Department of Aquatic Animal Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 2-Young Researchers and Elites Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran *Corresponding author's Email: mehdi.raissy@iaushk.ac.ir
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Abstract
Streptococcosis has been defined as a hemorrhagic septicemia in many fish species especially rainbow trout. This disease causes serious economic losses due to high mortality in fish and the bacteria responsible is also considered as a zoonotic pathogen. The aim of this study was identification of different causative agents of warm water streptococcosis and evaluation of the capsule gene inLactococcus garvieae isolates collected from rainbow trout in Chaharmahal - Bakhtiari Province, Iran. A total of 430 clinically suspected fish were collected from 43 rainbow trout farms. The samples were analyzed using biochemical methods and multiplex-PCR. The results indicated that samples of 36 fish farms (83.7%) were infected with one of the studied bacteria. The infection byL. garvieae was found to be more than that by other species (30 of 36 farms, 83%). Infection byS. iniae andS. uberis was found in nine and one fish farm, respectively. Presence of virulence genes was studied inL. garvieae isolates by PCR assay. According to the results 34 of 50 isolates (68%) contained the capsule gene. The results revealed high prevalence of virulent L. garvieae in rainbow trout farms which should be considered in preventional policies such as vaccine production and also in public health.
Keywords: Streptococcosis, Virulence gene, Rainbow trout, PCR, Iran
Author:Raissy M.1*; Sarshoughi M.1; Moumeni M.2
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