Isolation, identification and antimicrobial susceptibility of pathogenic Aeromonas media isolated from diseased Koi carp (Cyprinus carpio koi)
Description:1-College of Fisheries, Henan Normal University, Xinxiang, 453007, China 2-Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China *Corresponding author's email: a These authors contributed equally to this work
Aeromonas infections are the most common bacterial disease of cultured fish in China. In this study, a gram-negative bacillus was isolated from the liver of diseased koi carp (Cyprinus carpiokoi), and named strain KC-2. The results of morphological and biochemical tests, as well as phylogenetic analysis derived from16S rRNA andgyrB sequences indicated that the isolated strain KC-2 was highly identical to the knownAeromonas media ATCC 33907. Experimental infection assays were conducted, and pathogenicity was demonstrated in crucian carp (Carassius auratus) and zebrafish (Danio rerio). Antimicrobial susceptibility testing showed that the strain KC-2 was sensitive to cefalotin, cefixime, cefotaxime, gentamicin, netilmicin, azithromycin and chloramphenicol. This is the first report on the isolation and identification ofA. media from diseased, cultured koi fish. The results of the study will provide a scientific reference for prevention of bacterial disease of koi carp and identification ofA. media in fish.
Keywords: Koi carp,Aeromonas media, Isolation, Identification, Antimicrobial susceptibility testing
Author:Lü A.J. 1,2*; Hu X.C.2a; Li L.1a; Sun J.F.2; Song Y.J.1; Pei C.1; Zhang C.1; Kong X.H.1
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