The ability of marine Bacillus spp. isolated from fish gastrointestinal tract and culture pond sediment to inhibit growth of aquatic pathogenic bacteria
Description:1-Laboratory of Gut Microbiology, College of Animal Sciences and Technology, Nanjing Agricultural University, Nanjing 210095, PR China 2-Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China 3-Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China *Corresponding author’s Email:;
In this research, antagonistic activity of twoBacillus species isolated from digestive tract of marine fish and culture pond of sea cucumber was studied. The inhibitory activity ofBacillus spp. isolates against some common pathogenic bacteria of fish was assessed using the agar diffusion method. The strain ofB. subtilis G024 exhibited antimicrobial activity againstVibrio anguillarum,V. harveyi,V. vulnificus,Streptococcus sp. andStaphylococcus aureus; the isolate ofB. amyloliquefaciens N004 inhibited growth inV. anguillarum,V. campbellii,V. vulnificus,V. parahamolyticus,Edwardsiella tarda,Streptococcussp.,B. cereus. Scanning electron microscopy (SEM) investigation of indicator bacteria showed that cell morphologies were strongly affected by the cell-free supernatant of the twoBacillus spp. isolates. It is determined that the culture filtrates contained inhibitors against growth of some pathogenic bacteria with different degrees of inhibition, although none of the culture filtrates could inhibit the growth ofV. fluvialis,V. alginolyticus,V. splendidus. Based upon these characteristics, both of the antagonisticBacillus spp. isolates could be the potential probiotics used in the aquaculture production.
Keywords:Bacillus; Antagonistic activity; Scanning electron microscopy; Probiotic; Aquaculture
Author:Chen Y.1; Li J.2; Xiao P.3; Zhu W.1*; Mo Z. 2*
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