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Microsatellite DNA markers for analysis of genetic population structure of stellate sturgeon (Acipenser stellatus Pallas, 1771) in the North (Volga and Ural Rivers) and South Caspian Sea (Sefidrud and Gorganrud Rivers)
Description:1- Department of Marine Biology and Fisheries Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran, 2- Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO). POB: 14965-149. Tehran- Iran. * Corresponding author's Email: mnoroozi@toniau.ac.ir
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Abstract
This study represents a large-scale population genetic analysis of the stellate sturgeon,Acipenser stellatus, in the Caspian Sea. In total, 192 samples of adult stellate sturgeon were collected from four commercial catch stations in the Northern (Volga and Ural Rivers) and Southern Caspian Sea (estuary of Sefidrud and Gorganrod Rivers-Iran). Fifteen sets of microsatellite primers developed from lake sturgeon (Acipenser fulvescens) and shovelnose sturgeon (Scaphirhynchus platorynchus) were tested on genomic DNA. Ten primer sets (LS-19, 34, 54, 68, Spl-105, 104, 163, 170, 173, 113) revealing polymorphic loci were used to analyze the genetic variation found in adults of the stellate sturgeon populations. Analyses revealed that the average number of alleles per locus was 13.05 (range 8 to 18 alleles per locus in regions, Ne= 7.86). All sampled regions contained private alleles. The average observed and expected heterozygosities were 0.665 and 0.862, respectively. Deviations from Hardy-Weinberg equilibrium were seen in most cases. Average of Fis, Fit and Nm were 0.230, 0.261 and 7.498, respectively. Pairwise PopulationFST Values ranged from 0.019 to 0.035.FST,RST, and gene flow estimates in AMOVA indicated significant genetic differentiation among and regions, indicating that the populations were divergent. The genetic distance between populations indicates that the genetic difference among the studied populations is pronounced. These results together with highly significantRST of genotypic differences between these pairs of collections support the existence of different genetic populations along the Caspian Sea coast.
Keywords: Genetic variability, Genetic differentiation,Acipenser stellatus, Microsatellite markers
Author:Norouzi M. 1*; Pourkazemi M.2
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