Vol.15, No. 1,January2016
Transcriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) Uusing Illumina paired-end sequencing to identify genes and markers
Description:-Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo 315211, China 2-Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China 3-JinHua Polytechnic College, JinHua 321000, China 4- Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Huzhou 313001, China * Corresponding author's Email: lukaihong@nbu.edu.cn
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Abstract
The transcriptome of triangle sail musselHyriopsis cumingii (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonads, hepatopancreas, foot, mantel, gills and adductor muscles, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48% Q20 bases were generated. Clustering and assembly of these reads produced a non-redundant set of 92,347 unigenes with an average length of 1,150.61 bp. 11,174 unigenes were involved in the molecular function, cellular component and biological process categories by GO (Gene Ontology) analysis. Potential genes and their functions were predicted by KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway mapping and COG (Cluster of Orthologous Groups of proteins) analysis. More than 8 putative genes of interest involved in sex determination/differentiation were identified. Furthermore, 8,014 SSRs and 38,288 SNPs were identified in this transcriptome dataset.
Keywords:Hyriopsis cumingii, Illumina paired-end sequencing, Transcriptome, Sex determination, Molecular marker
Author:Zhang A. J.1,2,4; Liu S. L.2,4; Zhu J. Y.1;. Gu Z. M.2,4; Zhou Z. M.2,4; Zhang G. F.3; Lu K. H. 1*
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