Vol.15, No. 1,January2016
Effects of dietary Bacillus subtilis on growth performance and immune responses, in rainbow trout, Oncorhynchus mykiss (Walbaum, 1792)
Description:1- Faculty of veterinary medicine, Urmia University, Uremia, Iran 2- Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine and Artemia and Aquatic Animals Research Institute, Urmia University, Urmia, Iran 3- Pathobiology and Quality Control, Artemia and Aquatic Research Institute, Urmia University, Urmia, Iran 4- Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Science and research Branch, Tehran, Iran 5- Department of Food Sciences and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran * Corresponding author's Email: Mahmoudzadeh.fst85@gmail.com
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Abstract
Four hundred and eighty five rainbow trout (76 ± 6.44 g mean weight) were acclimated to laboratory conditions and then randomly divided into four groups of tanks in triplicate. The first group (Group 1) was fed on a commercial diet (control) withoutBacillus subtilis supplementation. The second, third and fourth groups (Group 2, Group 3 and Group 4, respectively) were given a diet supplemented with 1, 5 and 10 g probiotic powder (containing 8×107 CFU g-1Bacillus subtilis) per kg commercial feed. Growth performance, immune responses and glucose levels were analyzed on days 0, 22 and 44. The results showed that dietaryBacillus subtilis supplementation significantly (p<0.05) reduced final weight in treated groups compared with that in the control group. No significant difference (p> 0.05) was observed in weight gain rate (WGR) between the control and group 2. On the effect of dietaryBacillus subtilis on serum lysozyme, it was observed that group 3 and group 4 did not show any significant differences in serum lysozyme activity and serum total antibody on day 44. Results of alternative complement activity, showed significant increase during the experimental days (p< 0.05). Results on glucose assay showed that group 3 had the lowest glucose level (13.71 mg/dL) which was not significantly different than that in other groups on day 44. Fish diet supplementation with 5 g probiotic powder (Group 3) is preferable for immune system responses; however, high dose ofBacillus subtilis may be helpful to improve growth performance in rainbow trout,Oncorhynchus mykiss (Walbaum, 1792).
Keywords:Rainbow trout,Bacillus subtilis,Dietary supplementation, Growth factors, Immune function
Author:Mahmoudzadeh L. 1;Meshkini S. 2,Tukmehchi A.3; Motalebi Moghanjoghi A.A. 4; Mahmoudzadeh M. 5*
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