Vol. 14,No. 4, October 2015
Application of AFLP molecular marker for genetic analysis of black pomfret Parastromateus niger from the Persian Gulf The influence of ovarian fluid on the sperm physiology of Rutilus kutum Effect of dietary wood betony, Stachys lavandulifolia Vahl extract on growth performance, and haematological and biochemical parameters of Common carp, Cyprinus carpio Diet and trophic level of scalloped hammerhead shark (Sphyrna lewini) from the gulf of California and gulf of Tehuantepec, Mexico Bacillus
Description:1- Department of Fisheries, Faculty of Marine and Atmospheric Science and Technology, Hormozgan University, Bandar Abbas, Iran 2- Department of Biotechnology, Persian Gulf Research and Studies Center, Persian Gulf University, Bushehr, Iran *Corresponding author's email: sourinejad@hormozgan.ac.ir
Black pomfretParastromateus niger is a commercially important fishery resource in the Persian Gulf but harvesting its stocks lacks genetic identification of populations. AFLP technique was applied to analyze genetic diversity and population structure of 32 fish from coastal waters of Bandar Abbas, Bushehr and Abadan with 7EcoRI/MseI primer pair combinations. In total, 381 bands were produced of which, 46 were polymorphic (12.07%). Percentage of polymorphic bands was higher in Bushehr samples (91.30%) than in Abadan (84.78%) and Bandar Abbas (73.91%) samples. The highest level of heterozygosity based on Nei’s coefficient and Shannon’s index was observed in Bushehr fish (0.38±0.16 and 0.54±0.21). Observed and effective alleles ranged from 1.73±0.44 and 1.53±0.40 in Bandar Abbas samples to 1.91±0.28 and 1.70±0.34 in Bushehr samples. The averageFst was 0.19 indicating high genetic differentiation among the three locations. Gene flow with mean of 1.93 was the lowest level between Bandar Abbas and Abadan (1.24). Nei's genetic identity revealed the least genetic similarity between the samples of Bandar Abbas and Abadan (0.77). AMOVA analysis demonstrated 81% of the genetic variation within populations and 19% among populations. The UPGMA dendrogram clustered all 32 individuals into 3 groups. In some cases individuals from the same region were grouped together but in most cases, gene exchange was observed to be common among the groups. Analyses provided evidence for genetic differentiation among the three locations, indicating separate populations of black pomfret in the northern Persian Gulf.

Keywords: Black pomfret,Parastromateus niger, AFLP molecular markers, Population structure, Persian Gulf
Author:Sharifi M.1; Sourinejad I.1*; Hosseini S.J.2; Qasemi S.A.2
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