Vol. 14,No. 4, October 2015
Growth performance and disease resistance towards Aeromonas hydrophila in Hemibagrus nemurus (Valenciennes, 1840) fingerlings through probiotic feeding
Description:1-Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 2-Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia *Corresponding author’s email: cheroos@gmail.com
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Abstract
A study was carried out to evaluate the probiotic activity ofBacillus subtilisG1 isolated from fermented pickles in growth performance and disease resistance ofHemibagrus nemurus fingerlings at Universiti Putra Malaysia. The probiotic was mixed in feed at doses of 0 (C, control), 3 x 109 (T1) 3 x 107 (T2) and 3 x 105 (T3) cfu g-1 and fed to the catfish fingerlings for nine weeks. Results showed that catfish fed a diet containing 107 cfu g-1B. subtilis G1 had significantly higher percent weight gain (248.69 ± 3.31%), and better food conversion ratio (1.68 ± 0.03), than those of other treatments. Inhibitory activity of the probioticB. subtilis G1 against fish pathogensAeromonas hydrophila andStreptococcus agalactiae was evaluated by well diffusion agar method. Inhibition zones measured showedA. hydrophila andS. agalactiae were 16.13 ± 0.91 mm and 17.5 ± 1.84 mm, respectively, indicating strong inhibitory activity against the pathogens.Three weeks after the feeding trial, the fingerlings were challenged with 0.1 ml containing 106 cfu ml-1 ofA. hydrophila by intra-peritoneal injection. After 14 days, the mortality rate of catfish was significantly lower in group T1 (30 ± 5.8%) compared to the control (C) group (56.7 ± 3.3%). The findings of this study proved that administration ofB. subtilis G1 can improve growth and disease resistance in catfish.

Keywords:Hemibagrus nemurus, Probiotic, Growth performance, Disease resistance,Aeromonas hydrophila
Author:Farhana A.A.1; Saad C.R.1*; Kamarudin M.S.1; Daud H.M.2
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