Vol. 14,No. 1, January 2015
Isolation and identification of halophilicbacteria from Urmia Lake in Iran
Description: 1-Microbiology Department, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran. 2-Iranian Biological Resource Center 3-Microbiology Department, Faculty of Biology, College of Science, University of Tehran, Tehran, Iran. 4-Pathobiology and Quality Control Departmentt, Artemia and Aquatic Animals Institute, University of Urmia, Urmia, Iran. 5-Iranian Fisheries Research Organization, Tehran, Iran. *Corresponding author's email: akhavansepahy@gmail.com
Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to form major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake from 10 stations and depth of approximately 30-50 cm, in April 2011 and transfer to the laboratory in the sterile conditions, samples were enriched and cultured on defined media, and incubated. After appearance of colonies, selected strains were studied based on morphology, physiology and biochemical characteristics. For phylogenetic identification, their genomic DNA were extracted and amplified by PCR technique. Therefore their sequences were determined by genetic experiment based on 16S rRNA gene sequence and their similarity were analysed in GenBank of EzTaxon database. Finally the phylogenetic tree was constructed. Studied strains belonged to three genera: Halomonas 50% (including H. andesensis LC6(T)[12.5%], H. gomseomensis M12(T)[12.5%], H. hydrothermalis Slthf2(T)[12.5%], H. boliviensis LC1(T)[6.25%] and H. janggokensis M24(T)[6.25%]), Salinivibrio 25% (including S.costicolasubsp. alcaliphilus DSM 16359(T)[18.75%] and S. sharmensis BAG(T)[6.25%]) and Idiomarina 25% (including I .loihiensis L2TR(T)[25%]).

Monday 9 Nov 2015
Author: Irannejad S.1,2; AkhavanSepahy A.1,2*; Amoozegar M. A.2,3; Tukmechi A.4; Motallebi Moghanjoghi A.A.
keyword: Halophilic bacteria, Species diversity, Isolation, Phylogenetic, DNA analysis, Urmia Lake
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