Vol. 13,No. 2, April 2014
Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus va
Description: 1-Biology Department, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran 2-Microbiology Department, North of Tehran Branch, Islamic Azad University, Tehran, Iran 3-Iranian Fisheries Research Organization, P.O.Box: 14155-6116, Tehran, Iran *Corresponding author's email: maryam.mirbakhsh@gmail.com
A Gram-negative, rod-shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery-reared larval of Iran Shrimp Research Centerfollowing mass mortality . Seven isolates of Vibrio spp.were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate- Bile Sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O/129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1).

Monday 4 May 2015
Author: Mirbakhsh, M.*1; Akhavan sepahy, A.2; Afsharnasab, M3; Khanafari, A2; Razavi, M.R1
KeyWord: Vibriosis, shrimp diseases, Litopenaeus vannamei, 16S rDNA sequencing
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