Vol. 12,No. 4, October 2013
Isolation, identification and phylogenetic analysis of a pathogen of Haliotis diversicolor supertext
Description: 1-College of Life Science, QuFu Normal University, QuFu, China 2-College of Food Science and Light Industry, South China University of Technology, Guangzhou, China
This study was conducted to determine a disease outbreak in 14 day old post-larvae of abalone (Haliotis diversicolor supertexta) which caused mass mortality in July 2010 in Shanwei, China. Twenty-nine bacterial strains were isolated from a sample pool of 10 diseased post-larval abalones on 2216E marine agar plates during a natural outbreak of the disease. Among them, a dominant isolate (referred to as strain 21) was found to be highly virulent to post-larvae in experimental challenge tests, with an LD50 value of 1.0 ×104 colony forming units (CFU) mL-1 on day 3. API 20NE kits and 16S rDNA sequence analysis, identified strain 21 as Oceanomonas doudoroffii. It was susceptible to 10 and moderately susceptible to 1 of the 16 antibiotics examined when antibiotic sensitivities of the bacterium were assayed. Results of this study implicated Oceanomonas doudoroffii strain 21 as a cause of mortalities in post-larval abalone from Shanwei, China.

Thursday 7 May 2015
Author: Zhou Jing*1and Cai Junpeng 2
KeyWord: Haliotis diversicolor supertexta, massive death, challenge test, Oceanomonas doudoroffii, 16S rDNA s
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