Vol. 13,No. 1, January 2014
Pathogenicity of viral nervous necrosis virus for Guppy fish, Poecilia reticulata
Description: 1-Department of Biology, Falavarjan Branch, Isalmic Azad University, Esfahan, Iran. 2-Faculty of Veterinary Medicine, University of Putra. No. 43400 Serdang, Selangor, Malaysia. 3-Fish Pathology Department,OIE Reference Lab for Encephalopathy and Retinopathy, Institute Zooprofilattico Sperimentale delle Venezie, Viale dell'Università, 10- 35020 Legnaro, Padova- Italy. 4-Iranian Fisheries Research Organization, P.O.Box: 14155-6116 Tehran, Iran
Abstract: 
The pathogenicity of a Nervous Necrosis Virus isolate obtained from naturally infected Golden grey mullet (Liza auratus) suffering serious mortalities in Iranian coastline water of the Caspian Sea was investigated for first time. An experimental infection has been performed using three groups, two experimental groups and one control group of Guppy (Poecilia reticulata) with mean weight 0.47±0.09 g, at temperature 25ºC. The infectious dosage (50 ml) with TCID50/ml= 10 4.25for 2 hours in group 1and 4 hours in group 2 developed the disease with immersion method. Clear clinical signs associated with significant mortality were observed since 15 dpi. Cumulative mortalities rose to 100% at 30 dpi. While in the control group no mortality was recorded.
Virus was re-isolated on SSN-1 cell line that showing typical CPE developed after inoculation with tissues filtrate from dead fish. Histopathological examination of exposed fish, showed clear vacuolization in the granular layer of the retina and cerebellum. TEM micrographs revealed intracytoplasmic vacuoles in the retina of infected Guppy. IHC revealed the presence of viral antigens in the brain and retina.
These results confirmed the pathogenicity of the NNV isolate obtained from Golden grey mullet suffering high mortality with regard to suggest that the same agent isolated from golden grey mullet is very likely the cause of the mortality observed in the same species.

Thursday 7 May 2015
Author: Nazari, A.1٭; Hassan, M. D.2; Bovo, G.3; Zorriehzahra, M. J.4; Azmi,T. I.2and Arshad, S.S.2
KeyWord: VNN, Golden grey mullet, Caspian Sea
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