Vol. 13,No. 4,October 2014
Identification of a sex-linked SCAR marker for Plecoglossus altivelis and its application for identi
Description: Key laboratory of ministry of education applied marine biotechnology, Ningbi University, Ningbo 315211, China *Corresponding author's email: limingyun@nbu.edu.cn
Ayu (Plecoglossus altivelis), one kinds of valuable cultured fish species, show almost no morphological difference between male and female until sexual maturity. Here, we report the identification of sex-linked markers for the ayu, based on Amplified Fragment Length Polymorphism (AFLP) generated from cultured fish (15 males and 15 females) by using 63 different primer combinations. Genomic fragments (n= 3733) were produced with a mean frequency of 59 bands per primer pair. A male-specific of 139 base pair band was amplified and converted to a sequence-characterized amplified region (SCAR marker) designated as Ayu102. Six distinct genomic fragments were produced in 12 wild samples (6 males and 6 females). The fragments designated a, b, c, d, and e were detected only in males, and one fragment (f) was detected in both genders. Nucleotide sequence analysis showed that fragments e and f were 96.67% identical. Ayu102 marker was detected in 45-d-old larvae, in both cultured and wild fish populations, and in offsprings generated by gynogenesis. Fragment e was detected in all males, and in only 6.7% of females in cultured fish. Data suggest that Ayu102 marker is a male-specific marker linked to the sex-determining locus of the male ayu and can be used for gender identification.

Sunday 3 May 2015
Author: Wang C.; Li M.*; Wang J.; Tu Z.; Fan H.
KeyWords: AFLP, Plecoglossus altivelis, Sex-linked marker.
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